ssr_primer
Primer Details for TRINITY_DN85871_c7_g1_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTATCTGTACGGACCACC | AAAAGTCGCCGGCAAAGAAC | Start: 413 |
| Tm | 59.97 | 59.97 | End: 530 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TGCTCGATGCCATTTCACCT | AAAAGTCGCCGGCAAAGAAC | Start: 299 |
| Tm | 60.035 | 59.97 | End: 530 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TGCTCGATGCCATTTCACCT | GGTGGTCCGTACAGATAGCG | Start: 299 |
| Tm | 60.035 | 59.97 | End: 432 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | AGCTGCGCCTAAGAAACAGT | GGTGGTCCGTACAGATAGCG | Start: 166 |
| Tm | 59.965 | 59.97 | End: 432 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | CGGACCACCACCAATCTGAA | AAAAGTCGCCGGCAAAGAAC | Start: 424 |
| Tm | 59.964 | 59.97 | End: 530 |
| Size | 20 | 20 | 107 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTATCTGTACGGACCACC | AAAAGTCGCCGGCAAAGAAC | Start: 413 |
| Tm | 59.97 | 59.97 | End: 530 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TGCTCGATGCCATTTCACCT | AAAAGTCGCCGGCAAAGAAC | Start: 299 |
| Tm | 60.035 | 59.97 | End: 530 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TGCTCGATGCCATTTCACCT | GGTGGTCCGTACAGATAGCG | Start: 299 |
| Tm | 60.035 | 59.97 | End: 432 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | AGCTGCGCCTAAGAAACAGT | GGTGGTCCGTACAGATAGCG | Start: 166 |
| Tm | 59.965 | 59.97 | End: 432 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | CGGACCACCACCAATCTGAA | AAAAGTCGCCGGCAAAGAAC | Start: 424 |
| Tm | 59.964 | 59.97 | End: 530 |
| Size | 20 | 20 | 107 |
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