ssr_primer
Primer Details for TRINITY_DN85866_c0_g1_i4 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACCGGCAACAGCATTTGA | TGGAGCCTTGTATCCTCCGA | Start: 1126 |
| Tm | 59.968 | 60.033 | End: 1395 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
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| Sequence | CGGCAACAGCATTTGAAGCT | TGGAGCCTTGTATCCTCCGA | Start: 1130 |
| Tm | 60.039 | 60.033 | End: 1395 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | GCTCATGAACGACACGATGC | GTCGACCTGACTCAAGGAGC | Start: 407 |
| Tm | 59.974 | 60.109 | End: 527 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | GACAGGCTGAACTGCTTCCT | CCGCTAAGAACGCAGGAGAT | Start: 1776 |
| Tm | 59.964 | 59.897 | End: 1979 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | AGACAGGCTGAACTGCTTCC | CCGCTAAGAACGCAGGAGAT | Start: 1775 |
| Tm | 59.964 | 59.897 | End: 1979 |
| Size | 20 | 20 | 205 |
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