ssr_primer
Primer Details for TRINITY_DN85856_c1_g2_i2 |
|---|
| (A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTTTGGCTTCCCCTAGTT | ATGTTTGCAGGCGTTCAACC | Start: 5312 |
| Tm | 59.962 | 59.968 | End: 5449 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | GGTTGAACGCCTGCAAACAT | CCTCTAGAACACCAGCAGGC | Start: 5430 |
| Tm | 59.968 | 60.108 | End: 5704 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CCTCTAGAACACCAGCAGGC | Start: 5494 |
| Tm | 59.965 | 60.108 | End: 5704 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | GGGGAGGGGGACAAAAACAA | ATCCAACATGGCTGACAGCA | Start: 4236 |
| Tm | 60.105 | 59.961 | End: 4467 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CGCCACTGCTCCATTTCAAC | Start: 5494 |
| Tm | 59.965 | 60.11 | End: 5768 |
| Size | 20 | 20 | 275 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTTTGGCTTCCCCTAGTT | ATGTTTGCAGGCGTTCAACC | Start: 5312 |
| Tm | 59.962 | 59.968 | End: 5449 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | GGTTGAACGCCTGCAAACAT | CCTCTAGAACACCAGCAGGC | Start: 5430 |
| Tm | 59.968 | 60.108 | End: 5704 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CCTCTAGAACACCAGCAGGC | Start: 5494 |
| Tm | 59.965 | 60.108 | End: 5704 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | GGGGAGGGGGACAAAAACAA | ATCCAACATGGCTGACAGCA | Start: 4236 |
| Tm | 60.105 | 59.961 | End: 4467 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CGCCACTGCTCCATTTCAAC | Start: 5494 |
| Tm | 59.965 | 60.11 | End: 5768 |
| Size | 20 | 20 | 275 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTTTGGCTTCCCCTAGTT | ATGTTTGCAGGCGTTCAACC | Start: 5312 |
| Tm | 59.962 | 59.968 | End: 5449 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | GGTTGAACGCCTGCAAACAT | CCTCTAGAACACCAGCAGGC | Start: 5430 |
| Tm | 59.968 | 60.108 | End: 5704 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CCTCTAGAACACCAGCAGGC | Start: 5494 |
| Tm | 59.965 | 60.108 | End: 5704 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | GGGGAGGGGGACAAAAACAA | ATCCAACATGGCTGACAGCA | Start: 4236 |
| Tm | 60.105 | 59.961 | End: 4467 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGAGAATGTGTCTGCGCCAA | CGCCACTGCTCCATTTCAAC | Start: 5494 |
| Tm | 59.965 | 60.11 | End: 5768 |
| Size | 20 | 20 | 275 |
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