ssr_primer
Primer Details for TRINITY_DN85804_c4_g1_i1 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCTCGGAGGGTTTTCAGGG | GGAGGGACGGGAAACAAGAG | Start: 404 |
| Tm | 60.036 | 60.036 | End: 562 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | GGTAGCAATATCCCCCGTGG | CAAGAAGTGTCAACCGCTGC | Start: 1141 |
| Tm | 59.964 | 60.041 | End: 1280 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | AAAAAGGCCCGACCAGGAAA | Start: 543 |
| Tm | 60.036 | 60.106 | End: 756 |
| Size | 20 | 20 | 214 |
| Primer 4 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | ACCAGGAAACGGCGTAAGTT | Start: 543 |
| Tm | 60.036 | 59.893 | End: 745 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | GGAGGTGGATCCGTTCCTTG | Start: 543 |
| Tm | 60.036 | 60.108 | End: 791 |
| Size | 20 | 20 | 249 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCTCGGAGGGTTTTCAGGG | GGAGGGACGGGAAACAAGAG | Start: 404 |
| Tm | 60.036 | 60.036 | End: 562 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | GGTAGCAATATCCCCCGTGG | CAAGAAGTGTCAACCGCTGC | Start: 1141 |
| Tm | 59.964 | 60.041 | End: 1280 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | AAAAAGGCCCGACCAGGAAA | Start: 543 |
| Tm | 60.036 | 60.106 | End: 756 |
| Size | 20 | 20 | 214 |
| Primer 4 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | ACCAGGAAACGGCGTAAGTT | Start: 543 |
| Tm | 60.036 | 59.893 | End: 745 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | CTCTTGTTTCCCGTCCCTCC | GGAGGTGGATCCGTTCCTTG | Start: 543 |
| Tm | 60.036 | 60.108 | End: 791 |
| Size | 20 | 20 | 249 |
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