ssr_primer
Primer Details for TRINITY_DN85740_c1_g1_i3 |
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| (A)13ttattcaattcaataaccaatgttttcattttaaagattttttccaattt(A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTATCGGTCGTGATGTCA | CAATGGTTTCCGCCGGTTTT | Start: 1317 |
| Tm | 59.971 | 59.967 | End: 1581 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | CGCTATCGGTCGTGATGTCA | TCTCCGGCTTCCATGAGAGA | Start: 1317 |
| Tm | 59.971 | 60.033 | End: 1421 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGCTATCGGTCGTGATGTCA | CTTGGACGGCATCTGGAACT | Start: 1317 |
| Tm | 59.971 | 60.036 | End: 1465 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
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| Sequence | ATCCACACTTGGCAAGCGTA | TGACATCACGACCGATAGCG | Start: 1190 |
| Tm | 59.964 | 59.971 | End: 1336 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TATCCACACTTGGCAAGCGT | TGACATCACGACCGATAGCG | Start: 1189 |
| Tm | 59.964 | 59.971 | End: 1336 |
| Size | 20 | 20 | 148 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTATCGGTCGTGATGTCA | CAATGGTTTCCGCCGGTTTT | Start: 1317 |
| Tm | 59.971 | 59.967 | End: 1581 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | CGCTATCGGTCGTGATGTCA | TCTCCGGCTTCCATGAGAGA | Start: 1317 |
| Tm | 59.971 | 60.033 | End: 1421 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGCTATCGGTCGTGATGTCA | CTTGGACGGCATCTGGAACT | Start: 1317 |
| Tm | 59.971 | 60.036 | End: 1465 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | ATCCACACTTGGCAAGCGTA | TGACATCACGACCGATAGCG | Start: 1190 |
| Tm | 59.964 | 59.971 | End: 1336 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TATCCACACTTGGCAAGCGT | TGACATCACGACCGATAGCG | Start: 1189 |
| Tm | 59.964 | 59.971 | End: 1336 |
| Size | 20 | 20 | 148 |
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