ssr_primer
Primer Details for TRINITY_DN85736_c2_g1_i2 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGTAGGGCTGCTGACTA | AGGAGGAAGCGATCAGTCCT | Start: 2720 |
| Tm | 60.034 | 60.033 | End: 2895 |
| Size | 20 | 20 | 176 |
| Primer 2 details |
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| Sequence | AGTCGTAGGGCTGCTGACTA | AACAATGCAGGAGGAAGCGA | Start: 2720 |
| Tm | 60.034 | 59.963 | End: 2903 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
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| Sequence | GATTTGTGGCCCCTGGAAGA | AGGAGGAAGCGATCAGTCCT | Start: 2660 |
| Tm | 59.96 | 60.033 | End: 2895 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
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| Sequence | ATTTGTGGCCCCTGGAAGAG | AGGAGGAAGCGATCAGTCCT | Start: 2661 |
| Tm | 59.96 | 60.033 | End: 2895 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | AGGGCTGCTGACTAGGGTTA | AGGAGGAAGCGATCAGTCCT | Start: 2726 |
| Tm | 59.957 | 60.033 | End: 2895 |
| Size | 20 | 20 | 170 |
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