ssr_primer
Primer Details for TRINITY_DN85710_c1_g1_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCCAAGTCACGCGAAAAA | ATTCTGGAGCTACGCCCATG | Start: 252 |
| Tm | 59.971 | 59.893 | End: 463 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
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| Sequence | GCAGCTGACGGTTGTGATTG | TTTTTCGCGTGACTTGGCAG | Start: 108 |
| Tm | 60.11 | 59.971 | End: 271 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
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| Sequence | GCTGACGGTTGTGATTGAGC | TTTTTCGCGTGACTTGGCAG | Start: 111 |
| Tm | 59.833 | 59.971 | End: 271 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
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| Sequence | AGGACAGTTGATGGATCGCC | TTTTTCGCGTGACTTGGCAG | Start: 149 |
| Tm | 59.821 | 59.971 | End: 271 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | CGGTTGTGATTGAGCGCTTC | TTTTTCGCGTGACTTGGCAG | Start: 116 |
| Tm | 60.179 | 59.971 | End: 271 |
| Size | 20 | 20 | 156 |
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