ssr_primer
Primer Details for TRINITY_DN85703_c1_g5_i1 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAGAGCAGAGCTGAAGGC | TGACTTTTGCTTTGCGACCG | Start: 269 |
| Tm | 60.037 | 59.971 | End: 507 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TGTTGCTGAGATTGGCGACT | GCCTTCAGCTCTGCTCTGAA | Start: 147 |
| Tm | 59.965 | 60.037 | End: 288 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TTCAGAGCAGAGCTGAAGGC | GAGGGGTTGAATCACAGCGA | Start: 269 |
| Tm | 60.037 | 60.036 | End: 488 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | GGTGGTCCAATTCTGCCTCA | GCCTTCAGCTCTGCTCTGAA | Start: 72 |
| Tm | 59.962 | 60.037 | End: 288 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GCTGAAGGCTCTCTACCGAC | TGACTTTTGCTTTGCGACCG | Start: 280 |
| Tm | 59.898 | 59.971 | End: 507 |
| Size | 20 | 20 | 228 |
(AAGA)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAGAGCAGAGCTGAAGGC | TGACTTTTGCTTTGCGACCG | Start: 269 |
| Tm | 60.037 | 59.971 | End: 507 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TGTTGCTGAGATTGGCGACT | GCCTTCAGCTCTGCTCTGAA | Start: 147 |
| Tm | 59.965 | 60.037 | End: 288 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TTCAGAGCAGAGCTGAAGGC | GAGGGGTTGAATCACAGCGA | Start: 269 |
| Tm | 60.037 | 60.036 | End: 488 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | GGTGGTCCAATTCTGCCTCA | GCCTTCAGCTCTGCTCTGAA | Start: 72 |
| Tm | 59.962 | 60.037 | End: 288 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GCTGAAGGCTCTCTACCGAC | TGACTTTTGCTTTGCGACCG | Start: 280 |
| Tm | 59.898 | 59.971 | End: 507 |
| Size | 20 | 20 | 228 |
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