ssr_primer
Primer Details for TRINITY_DN85700_c3_g1_i10 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCTGTTTGACAACTCGCC | TGCATTAAGGTGCCTCTGGG | Start: 442 |
| Tm | 59.692 | 60.034 | End: 562 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
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| Sequence | CCCCCAAAACAAAATGGCGT | GAGGGTGGGCTCAACTGAAG | Start: 931 |
| Tm | 59.892 | 60.322 | End: 1118 |
| Size | 20 | 20 | 188 |
| Primer 3 details |
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| Sequence | CCCCCAAAACAAAATGGCGT | AGAATGGGGAGAGAGGGTGG | Start: 931 |
| Tm | 59.892 | 60.327 | End: 1130 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
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| Sequence | GAGTGAGTGTGCGAGCTCTC | TGCATTAAGGTGCCTCTGGG | Start: 342 |
| Tm | 60.456 | 60.034 | End: 562 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | ATACGGCGTTTACCTTGGCA | GAGAGCTCGCACACTCACTC | Start: 120 |
| Tm | 60.036 | 60.456 | End: 361 |
| Size | 20 | 20 | 242 |
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