ssr_primer
Primer Details for TRINITY_DN85693_c9_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGAAAGCTGGGGAATTTC | TGGGAAAAGGCATTCAGTGG | Start: 36 |
| Tm | 59.182 | 58.369 | End: 166 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | GCGGGAAAGCTGGGGAATTT | TGGGAAAAGGCATTCAGTGG | Start: 35 |
| Tm | 61.258 | 58.369 | End: 166 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGGGAAAGCTGGGGAATTTC | ATGGGAAAAGGCATTCAGTGG | Start: 36 |
| Tm | 59.182 | 58.818 | End: 167 |
| Size | 20 | 21 | 132 |
| Primer 4 details |
|---|
| Sequence | AGCTGGGGAATTTCTGACACA | TGGGAAAAGGCATTCAGTGG | Start: 42 |
| Tm | 59.575 | 58.369 | End: 166 |
| Size | 21 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | CGGGAAAGCTGGGGAATTTC | GCATTCAGTGGCTGGTTAAGAG | Start: 36 |
| Tm | 59.182 | 59.579 | End: 157 |
| Size | 20 | 22 | 122 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGAAAGCTGGGGAATTTC | TGGGAAAAGGCATTCAGTGG | Start: 36 |
| Tm | 59.182 | 58.369 | End: 166 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | GCGGGAAAGCTGGGGAATTT | TGGGAAAAGGCATTCAGTGG | Start: 35 |
| Tm | 61.258 | 58.369 | End: 166 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CGGGAAAGCTGGGGAATTTC | ATGGGAAAAGGCATTCAGTGG | Start: 36 |
| Tm | 59.182 | 58.818 | End: 167 |
| Size | 20 | 21 | 132 |
| Primer 4 details |
|---|
| Sequence | AGCTGGGGAATTTCTGACACA | TGGGAAAAGGCATTCAGTGG | Start: 42 |
| Tm | 59.575 | 58.369 | End: 166 |
| Size | 21 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | CGGGAAAGCTGGGGAATTTC | GCATTCAGTGGCTGGTTAAGAG | Start: 36 |
| Tm | 59.182 | 59.579 | End: 157 |
| Size | 20 | 22 | 122 |
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