ssr_primer
Primer Details for TRINITY_DN85675_c3_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGACCTTAGGGCCAGTGC | GGAAGTAAATACCCGCCCCC | Start: 103 |
| Tm | 60.034 | 60.179 | End: 264 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | GGGGGCGGGTATTTACTTCC | CAATGTGGGAGAGGGTGTCC | Start: 245 |
| Tm | 60.179 | 60.035 | End: 468 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
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| Sequence | GGGGGCGGGTATTTACTTCC | TGTGGGAGAGGGTGTCCTAG | Start: 245 |
| Tm | 60.179 | 59.959 | End: 465 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
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| Sequence | GGACACCCTCTCCCACATTG | CGAGGTGGTTTGTAGCGTGA | Start: 449 |
| Tm | 60.035 | 60.319 | End: 628 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
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| Sequence | GGACACCCTCTCCCACATTG | TCGAGGTGGTTTGTAGCGTG | Start: 449 |
| Tm | 60.035 | 60.319 | End: 629 |
| Size | 20 | 20 | 181 |
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