ssr_primer
Primer Details for TRINITY_DN85674_c0_g1_i5 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACATCACCGTTGGACAG | AAAAGGACGCCACCGAGAAT | Start: 2148 |
| Tm | 60.039 | 59.964 | End: 2380 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | TCGACATCACCGTTGGACAG | GTGGAACGTCTGGAGAGTGG | Start: 2148 |
| Tm | 60.039 | 60.038 | End: 2425 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | TCGACATCACCGTTGGACAG | GGTGGAACGTCTGGAGAGTG | Start: 2148 |
| Tm | 60.039 | 60.038 | End: 2426 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | TTTTTAGCGCGTTTGGAGGC | CTGTCCAACGGTGATGTCGA | Start: 2061 |
| Tm | 60.04 | 60.039 | End: 2167 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | TATGTGAGGGAGGCCGTACA | CACCACGTTGTTTTGGGTCC | Start: 76 |
| Tm | 60.033 | 59.898 | End: 335 |
| Size | 20 | 20 | 260 |
(T)40: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACATCACCGTTGGACAG | AAAAGGACGCCACCGAGAAT | Start: 2148 |
| Tm | 60.039 | 59.964 | End: 2380 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | TCGACATCACCGTTGGACAG | GTGGAACGTCTGGAGAGTGG | Start: 2148 |
| Tm | 60.039 | 60.038 | End: 2425 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | TCGACATCACCGTTGGACAG | GGTGGAACGTCTGGAGAGTG | Start: 2148 |
| Tm | 60.039 | 60.038 | End: 2426 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | TTTTTAGCGCGTTTGGAGGC | CTGTCCAACGGTGATGTCGA | Start: 2061 |
| Tm | 60.04 | 60.039 | End: 2167 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | TATGTGAGGGAGGCCGTACA | CACCACGTTGTTTTGGGTCC | Start: 76 |
| Tm | 60.033 | 59.898 | End: 335 |
| Size | 20 | 20 | 260 |
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