ssr_primer
Primer Details for TRINITY_DN85665_c1_g3_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACAAGGAATTGTTGCGG | ATCACAAGCAAGTGGTCGGT | Start: 310 |
| Tm | 59.968 | 59.891 | End: 411 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | AAACAGTGGTCCGGAATGCT | AAAACATTCCCGGGCGATCT | Start: 646 |
| Tm | 59.89 | 60.034 | End: 823 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGATCGCCCGGGAATGTTTT | TGGTCCATCTCAAGGGAGGT | Start: 804 |
| Tm | 60.034 | 59.881 | End: 967 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CGGAATGCTGCGTGAAATGT | AAAACATTCCCGGGCGATCT | Start: 657 |
| Tm | 59.831 | 60.034 | End: 823 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACCTTTAAACCCGGCAGCTT | GTGCTCAAACACACAGCCTG | Start: 1678 |
| Tm | 60.179 | 59.97 | End: 1902 |
| Size | 20 | 20 | 225 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACAAGGAATTGTTGCGG | ATCACAAGCAAGTGGTCGGT | Start: 310 |
| Tm | 59.968 | 59.891 | End: 411 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | AAACAGTGGTCCGGAATGCT | AAAACATTCCCGGGCGATCT | Start: 646 |
| Tm | 59.89 | 60.034 | End: 823 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGATCGCCCGGGAATGTTTT | TGGTCCATCTCAAGGGAGGT | Start: 804 |
| Tm | 60.034 | 59.881 | End: 967 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CGGAATGCTGCGTGAAATGT | AAAACATTCCCGGGCGATCT | Start: 657 |
| Tm | 59.831 | 60.034 | End: 823 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACCTTTAAACCCGGCAGCTT | GTGCTCAAACACACAGCCTG | Start: 1678 |
| Tm | 60.179 | 59.97 | End: 1902 |
| Size | 20 | 20 | 225 |
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