ssr_primer
Primer Details for TRINITY_DN85605_c2_g3_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCCAAATTATAGGCCCGA | TCATACGACGTTTTTGCGGC | Start: 45 |
| Tm | 58.945 | 59.836 | End: 213 |
| Size | 21 | 20 | 169 |
| Primer 2 details |
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| Sequence | AGCTCCAAATTATAGGCCCGA | CAGTGGGGGCCTTATCGAAT | Start: 45 |
| Tm | 58.945 | 59.526 | End: 187 |
| Size | 21 | 20 | 143 |
| Primer 3 details |
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| Sequence | AGCTCCAAATTATAGGCCCGAA | TCATACGACGTTTTTGCGGC | Start: 45 |
| Tm | 59.558 | 59.836 | End: 213 |
| Size | 22 | 20 | 169 |
| Primer 4 details |
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| Sequence | AAGCTCCAAATTATAGGCCCGA | TCATACGACGTTTTTGCGGC | Start: 44 |
| Tm | 59.558 | 59.836 | End: 213 |
| Size | 22 | 20 | 170 |
| Primer 5 details |
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| Sequence | GCTCCAAATTATAGGCCCGA | TCATACGACGTTTTTGCGGC | Start: 46 |
| Tm | 57.439 | 59.836 | End: 213 |
| Size | 20 | 20 | 168 |
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