ssr_primer
Primer Details for TRINITY_DN85586_c0_g2_i6 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCCTCTTCGACTTTGCC | CGACGCTCTTCCGATCTCTC | Start: 448 |
| Tm | 60.251 | 60.042 | End: 605 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | AACCCCTCTTCGACTTTGCC | TCACTCGCTGCCGACTTTAG | Start: 448 |
| Tm | 60.251 | 60.109 | End: 561 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | CCCCCGAGTAAGTAACCCCT | CGACGCTCTTCCGATCTCTC | Start: 435 |
| Tm | 60.326 | 60.042 | End: 605 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | AACCCCTCTTCGACTTTGCC | CATTTTCACTCGCTGCCGAC | Start: 448 |
| Tm | 60.251 | 60.179 | End: 566 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CCCTCTTCGACTTTGCCCTC | CGACGCTCTTCCGATCTCTC | Start: 451 |
| Tm | 60.392 | 60.042 | End: 605 |
| Size | 20 | 20 | 155 |
(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCCTCTTCGACTTTGCC | CGACGCTCTTCCGATCTCTC | Start: 448 |
| Tm | 60.251 | 60.042 | End: 605 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | AACCCCTCTTCGACTTTGCC | TCACTCGCTGCCGACTTTAG | Start: 448 |
| Tm | 60.251 | 60.109 | End: 561 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | CCCCCGAGTAAGTAACCCCT | CGACGCTCTTCCGATCTCTC | Start: 435 |
| Tm | 60.326 | 60.042 | End: 605 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | AACCCCTCTTCGACTTTGCC | CATTTTCACTCGCTGCCGAC | Start: 448 |
| Tm | 60.251 | 60.179 | End: 566 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CCCTCTTCGACTTTGCCCTC | CGACGCTCTTCCGATCTCTC | Start: 451 |
| Tm | 60.392 | 60.042 | End: 605 |
| Size | 20 | 20 | 155 |
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