Primer Details for TRINITY_DN85573_c2_g2_i2 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCGCTTGGGAAAGGTTTT | ACGTTACTTTCTTCTTTTCATTCATCT | Start: 18 |
| Tm | 58.23 | 57.943 | End: 239 |
| Size | 20 | 27 | 222 |
| Primer 2 details |
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| Sequence | ATGTCGCTTGGGAAAGGTTT | ACGTTACTTTCTTCTTTTCATTCATCT | Start: 17 |
| Tm | 58.005 | 57.943 | End: 239 |
| Size | 20 | 27 | 223 |
| Primer 3 details |
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| Sequence | AATGTCGCTTGGGAAAGGTT | ACGTTACTTTCTTCTTTTCATTCATCT | Start: 16 |
| Tm | 58.005 | 57.943 | End: 239 |
| Size | 20 | 27 | 224 |
| Primer 4 details |
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| Sequence | TGTCGCTTGGGAAAGGTTTT | GAAACGTTACTTTCTTCTTTTCATTCA | Start: 18 |
| Tm | 58.23 | 57.502 | End: 242 |
| Size | 20 | 27 | 225 |
| Primer 5 details |
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| Sequence | TGTCGCTTGGGAAAGGTTTT | TGAAACGTTACTTTCTTCTTTTCATTC | Start: 18 |
| Tm | 58.23 | 57.502 | End: 243 |
| Size | 20 | 27 | 226 |
