ssr_primer
Primer Details for TRINITY_DN85560_c0_g3_i2 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGGGAGGCGCTTAATCTT | GCAGCAGTTAGGGCCTGTTA | Start: 43 |
| Tm | 60.179 | 60.035 | End: 172 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | GACGGGAGGCGCTTAATCTT | AGAGTGCCTGAATGTGGACG | Start: 43 |
| Tm | 60.179 | 60.037 | End: 251 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
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| Sequence | CCCCCACGTGTTTTCGAATG | AGAGTGCCTGAATGTGGACG | Start: 133 |
| Tm | 59.76 | 60.037 | End: 251 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | GACGGGAGGCGCTTAATCTT | GCCTGAATGTGGACGGATGA | Start: 43 |
| Tm | 60.179 | 60.108 | End: 246 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | GACGGGAGGCGCTTAATCTT | GTGAGAGGGGCAGCAGTTAG | Start: 43 |
| Tm | 60.179 | 60.108 | End: 181 |
| Size | 20 | 20 | 139 |
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