ssr_primer
Primer Details for TRINITY_DN85522_c2_g7_i1 |
|---|
| (G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATTCTTTGCGTCGGCGTG | AGACGTCGACTCTAAGCCCT | Start: 1957 |
| Tm | 59.975 | 60.035 | End: 2190 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
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| Sequence | AGGGCTTAGAGTCGACGTCT | TGCAAGAAGTCGTCACCCTC | Start: 2171 |
| Tm | 60.035 | 59.966 | End: 2392 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | GCTCAGCAGAGAGTTGTCGT | AGACGTCGACTCTAAGCCCT | Start: 2015 |
| Tm | 60.039 | 60.035 | End: 2190 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
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| Sequence | AGGGCTTAGAGTCGACGTCT | CGGCCGAAGAAAATGTGCAA | Start: 2171 |
| Tm | 60.035 | 60.04 | End: 2407 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
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| Sequence | CAGCAGAGAGTTGTCGTCGT | AGACGTCGACTCTAAGCCCT | Start: 2018 |
| Tm | 60.04 | 60.035 | End: 2190 |
| Size | 20 | 20 | 173 |
(GAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATTCTTTGCGTCGGCGTG | AGACGTCGACTCTAAGCCCT | Start: 1957 |
| Tm | 59.975 | 60.035 | End: 2190 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | AGGGCTTAGAGTCGACGTCT | TGCAAGAAGTCGTCACCCTC | Start: 2171 |
| Tm | 60.035 | 59.966 | End: 2392 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | GCTCAGCAGAGAGTTGTCGT | AGACGTCGACTCTAAGCCCT | Start: 2015 |
| Tm | 60.039 | 60.035 | End: 2190 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | AGGGCTTAGAGTCGACGTCT | CGGCCGAAGAAAATGTGCAA | Start: 2171 |
| Tm | 60.035 | 60.04 | End: 2407 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | CAGCAGAGAGTTGTCGTCGT | AGACGTCGACTCTAAGCCCT | Start: 2018 |
| Tm | 60.04 | 60.035 | End: 2190 |
| Size | 20 | 20 | 173 |
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