ssr_primer
Primer Details for TRINITY_DN85512_c8_g3_i2 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGAAGGAGAGAAGCCAGTT | AAAAATTCGCCGAACTCCGC | Start: 308 |
| Tm | 60.037 | 60.11 | End: 447 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | GCGAAGGAGAGAAGCCAGTT | CCACCATGTCTGGACCACAA | Start: 308 |
| Tm | 60.037 | 59.89 | End: 572 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
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| Sequence | TATATTCCGCGGAGTTCGGC | CCACCATGTCTGGACCACAA | Start: 420 |
| Tm | 60.039 | 59.89 | End: 572 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
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| Sequence | GGTAGCGAAGGAGAGAAGCC | AAAAATTCGCCGAACTCCGC | Start: 304 |
| Tm | 59.897 | 60.11 | End: 447 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
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| Sequence | GGTAGCGAAGGAGAGAAGCC | CCACCATGTCTGGACCACAA | Start: 304 |
| Tm | 59.897 | 59.89 | End: 572 |
| Size | 20 | 20 | 269 |
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