ssr_primer
Primer Details for TRINITY_DN85507_c3_g5_i1 |
|---|
| (A)10gtgaactcatagagagatgcaagctgtatgctcaaattatttgttattagtattgctctcaggaaaaattacgcctttatcagtttgtttc(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCTTCCCCCTTCCTCCTT | AAGGTTACCCGATGGCATGG | Start: 1695 |
| Tm | 60.103 | 60.107 | End: 1806 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | GCTACCGCACAGTTATCCCA | AAGGAGGAAGGGGGAAGGTT | Start: 1492 |
| Tm | 59.823 | 60.103 | End: 1714 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | AACCTTCCCCCTTCCTCCTT | GTGCTGTGCGCTTCAGTTTT | Start: 1695 |
| Tm | 60.103 | 60.249 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
|---|
| Sequence | GGGCCCAGCTTGTATGCATA | TGGGATAACTGTGCGGTAGC | Start: 1244 |
| Tm | 60.179 | 59.823 | End: 1511 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | GCTTATGAGCCCCAAGAGCA | AGTCTCCGACATTGGGAAGC | Start: 550 |
| Tm | 60.107 | 59.749 | End: 649 |
| Size | 20 | 20 | 100 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCTTCCCCCTTCCTCCTT | AAGGTTACCCGATGGCATGG | Start: 1695 |
| Tm | 60.103 | 60.107 | End: 1806 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | GCTACCGCACAGTTATCCCA | AAGGAGGAAGGGGGAAGGTT | Start: 1492 |
| Tm | 59.823 | 60.103 | End: 1714 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | AACCTTCCCCCTTCCTCCTT | GTGCTGTGCGCTTCAGTTTT | Start: 1695 |
| Tm | 60.103 | 60.249 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
|---|
| Sequence | GGGCCCAGCTTGTATGCATA | TGGGATAACTGTGCGGTAGC | Start: 1244 |
| Tm | 60.179 | 59.823 | End: 1511 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | GCTTATGAGCCCCAAGAGCA | AGTCTCCGACATTGGGAAGC | Start: 550 |
| Tm | 60.107 | 59.749 | End: 649 |
| Size | 20 | 20 | 100 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCTTCCCCCTTCCTCCTT | AAGGTTACCCGATGGCATGG | Start: 1695 |
| Tm | 60.103 | 60.107 | End: 1806 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | GCTACCGCACAGTTATCCCA | AAGGAGGAAGGGGGAAGGTT | Start: 1492 |
| Tm | 59.823 | 60.103 | End: 1714 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | AACCTTCCCCCTTCCTCCTT | GTGCTGTGCGCTTCAGTTTT | Start: 1695 |
| Tm | 60.103 | 60.249 | End: 1927 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
|---|
| Sequence | GGGCCCAGCTTGTATGCATA | TGGGATAACTGTGCGGTAGC | Start: 1244 |
| Tm | 60.179 | 59.823 | End: 1511 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | GCTTATGAGCCCCAAGAGCA | AGTCTCCGACATTGGGAAGC | Start: 550 |
| Tm | 60.107 | 59.749 | End: 649 |
| Size | 20 | 20 | 100 |
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