Primer Details for TRINITY_DN85482_c5_g8_i1 | |||
|---|---|---|---|
| (T)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCACGGTGTGTCTTTTTA | TGAACGATTTATGCCTTACTTTGCT | Start: 12 |
| Tm | 59.061 | 59.586 | End: 290 |
| Size | 20 | 25 | 279 |
| Primer 2 details | |||
| Sequence | CCGCACGGTGTGTCTTTTTA | TGAACGATTTATGCCTTACTTTGC | Start: 12 |
| Tm | 59.061 | 58.363 | End: 290 |
| Size | 20 | 24 | 279 |
| Primer 3 details | |||
| Sequence | CTAACAATCTCCCCGCACGG | TGCTTTAAATCTGTTTTCCTGCT | Start: 0 |
| Tm | 60.81 | 57.21 | End: 269 |
| Size | 20 | 23 | 270 |
| Primer 4 details | |||
| Sequence | CCGCACGGTGTGTCTTTTTA | CTGAACGATTTATGCCTTACTTTGC | Start: 12 |
| Tm | 59.061 | 59.261 | End: 291 |
| Size | 20 | 25 | 280 |
| Primer 5 details | |||
| Sequence | CCGCACGGTGTGTCTTTTTA | TGCTTTAAATCTGTTTTCCTGCT | Start: 12 |
| Tm | 59.061 | 57.21 | End: 269 |
| Size | 20 | 23 | 258 |
