ssr_primer
Primer Details for TRINITY_DN85472_c1_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAAGCGGATCAGCGGACTC | TTGCTTTTCGCGGTCATTCG | Start: 54 |
| Tm | 59.898 | 60.11 | End: 291 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | AGCGGACTCGTGGAACTTTT | TTGCTTTTCGCGGTCATTCG | Start: 65 |
| Tm | 59.894 | 60.11 | End: 291 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | GGCCACTGAGCAAGGTACAA | TGCGTTGGAGTGGATTTCCA | Start: 3501 |
| Tm | 60.251 | 59.89 | End: 3610 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | ATACTGGGCCACTGAGCAAG | TGCGTTGGAGTGGATTTCCA | Start: 3495 |
| Tm | 59.746 | 59.89 | End: 3610 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
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| Sequence | TTCCACAAGTGAACTCGGCA | ACGCCGAGAAATAGTGCTGT | Start: 2946 |
| Tm | 59.821 | 59.753 | End: 3101 |
| Size | 20 | 20 | 156 |
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