ssr_primer
Primer Details for TRINITY_DN85471_c0_g2_i9 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAGGACAACTAGGCTTCA | GTTGCAGGCCAACGCTTAAA | Start: 1702 |
| Tm | 60.037 | 59.969 | End: 1958 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | TCAGTGAACCTGCTTGCGAT | CAACTGGGCGATTCCTCAGT | Start: 2667 |
| Tm | 59.965 | 60.036 | End: 2793 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TGCCAAGAGTAAGCTGTGGG | CAACTGGGCGATTCCTCAGT | Start: 2608 |
| Tm | 59.963 | 60.036 | End: 2793 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCCAAGAGTAAGCTGTGGGT | CAACTGGGCGATTCCTCAGT | Start: 2609 |
| Tm | 59.963 | 60.036 | End: 2793 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | ACCGCTTGACGCCTAAAAGA | TGGCGGCCGTAACATTTTTG | Start: 5146 |
| Tm | 59.966 | 60.039 | End: 5330 |
| Size | 20 | 20 | 185 |
(C)32: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAGGACAACTAGGCTTCA | GTTGCAGGCCAACGCTTAAA | Start: 1702 |
| Tm | 60.037 | 59.969 | End: 1958 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | TCAGTGAACCTGCTTGCGAT | CAACTGGGCGATTCCTCAGT | Start: 2667 |
| Tm | 59.965 | 60.036 | End: 2793 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TGCCAAGAGTAAGCTGTGGG | CAACTGGGCGATTCCTCAGT | Start: 2608 |
| Tm | 59.963 | 60.036 | End: 2793 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCCAAGAGTAAGCTGTGGGT | CAACTGGGCGATTCCTCAGT | Start: 2609 |
| Tm | 59.963 | 60.036 | End: 2793 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | ACCGCTTGACGCCTAAAAGA | TGGCGGCCGTAACATTTTTG | Start: 5146 |
| Tm | 59.966 | 60.039 | End: 5330 |
| Size | 20 | 20 | 185 |
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