ssr_primer
Primer Details for TRINITY_DN85428_c4_g4_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTGCAGAGAGAAGACCG | TGGAAAGGGTTGCACTCCAA | Start: 138 |
| Tm | 60.038 | 59.74 | End: 370 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | GCTGCAGAGAGAAGACCGAA | TGGAAAGGGTTGCACTCCAA | Start: 140 |
| Tm | 59.755 | 59.74 | End: 370 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCAGAGAGAAGACCG | ATGGAAAGGGTTGCACTCCA | Start: 138 |
| Tm | 60.038 | 59.518 | End: 371 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | ATCCAGCAATGGCCCAGATT | Start: 351 |
| Tm | 59.74 | 59.736 | End: 497 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | TCCAGCAATGGCCCAGATTC | Start: 351 |
| Tm | 59.74 | 60.396 | End: 496 |
| Size | 20 | 20 | 146 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTGCAGAGAGAAGACCG | TGGAAAGGGTTGCACTCCAA | Start: 138 |
| Tm | 60.038 | 59.74 | End: 370 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | GCTGCAGAGAGAAGACCGAA | TGGAAAGGGTTGCACTCCAA | Start: 140 |
| Tm | 59.755 | 59.74 | End: 370 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCAGAGAGAAGACCG | ATGGAAAGGGTTGCACTCCA | Start: 138 |
| Tm | 60.038 | 59.518 | End: 371 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | ATCCAGCAATGGCCCAGATT | Start: 351 |
| Tm | 59.74 | 59.736 | End: 497 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | TCCAGCAATGGCCCAGATTC | Start: 351 |
| Tm | 59.74 | 60.396 | End: 496 |
| Size | 20 | 20 | 146 |
(T)10accttgatcttggtttcataagatttttttacatttttttatttttttttctgctattattattacgttttcagggaatttttttta(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTGCAGAGAGAAGACCG | TGGAAAGGGTTGCACTCCAA | Start: 138 |
| Tm | 60.038 | 59.74 | End: 370 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | GCTGCAGAGAGAAGACCGAA | TGGAAAGGGTTGCACTCCAA | Start: 140 |
| Tm | 59.755 | 59.74 | End: 370 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCAGAGAGAAGACCG | ATGGAAAGGGTTGCACTCCA | Start: 138 |
| Tm | 60.038 | 59.518 | End: 371 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | ATCCAGCAATGGCCCAGATT | Start: 351 |
| Tm | 59.74 | 59.736 | End: 497 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | TTGGAGTGCAACCCTTTCCA | TCCAGCAATGGCCCAGATTC | Start: 351 |
| Tm | 59.74 | 60.396 | End: 496 |
| Size | 20 | 20 | 146 |
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