ssr_primer
Primer Details for TRINITY_DN85414_c5_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGACTCTTAAATCCGGCG | GGTAAAACCGAACAGTCTCCG | Start: 33 |
| Tm | 59.864 | 58.936 | End: 132 |
| Size | 21 | 21 | 100 |
| Primer 2 details |
|---|
| Sequence | AGCGGATTTTGCATTGAAACA | GTCTCCGTGATCAGGAACCG | Start: 8 |
| Tm | 57.616 | 60.179 | End: 118 |
| Size | 21 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGAGACTGTTCGGTTTTACC | GGCGACAAAATGAATGTGGGA | Start: 112 |
| Tm | 58.936 | 59.456 | End: 391 |
| Size | 21 | 21 | 280 |
| Primer 4 details |
|---|
| Sequence | CAAGCGGATTTTGCATTGAAACA | GTCTCCGTGATCAGGAACCG | Start: 6 |
| Tm | 59.506 | 60.179 | End: 118 |
| Size | 23 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | CCAGACTCTTAAATCCGGCGA | TGGTAAAACCGAACAGTCTCCG | Start: 34 |
| Tm | 59.864 | 60.546 | End: 133 |
| Size | 21 | 22 | 100 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGACTCTTAAATCCGGCG | GGTAAAACCGAACAGTCTCCG | Start: 33 |
| Tm | 59.864 | 58.936 | End: 132 |
| Size | 21 | 21 | 100 |
| Primer 2 details |
|---|
| Sequence | AGCGGATTTTGCATTGAAACA | GTCTCCGTGATCAGGAACCG | Start: 8 |
| Tm | 57.616 | 60.179 | End: 118 |
| Size | 21 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | CGGAGACTGTTCGGTTTTACC | GGCGACAAAATGAATGTGGGA | Start: 112 |
| Tm | 58.936 | 59.456 | End: 391 |
| Size | 21 | 21 | 280 |
| Primer 4 details |
|---|
| Sequence | CAAGCGGATTTTGCATTGAAACA | GTCTCCGTGATCAGGAACCG | Start: 6 |
| Tm | 59.506 | 60.179 | End: 118 |
| Size | 23 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | CCAGACTCTTAAATCCGGCGA | TGGTAAAACCGAACAGTCTCCG | Start: 34 |
| Tm | 59.864 | 60.546 | End: 133 |
| Size | 21 | 22 | 100 |
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