Primer Details for TRINITY_DN85402_c3_g1_i1 |
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| (A)10tttctattgaaaaaattattttattaattttgatggagtaattttaaatcatttattcgtgctaaaataacgttttac(T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACTAGTTTTGAGTGTGTGCT | AAAAGTAAAACGTTATTTTAGCACGAA | Start: 3 |
| Tm | 57.592 | 57.499 | End: 171 |
| Size | 22 | 27 | 169 |
| Primer 2 details |
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| Sequence | AGACTAGTTTTGAGTGTGTGCT | AAAAAGTAAAACGTTATTTTAGCACGA | Start: 3 |
| Tm | 57.592 | 57.499 | End: 172 |
| Size | 22 | 27 | 170 |
| Primer 3 details |
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| Sequence | AGACTAGTTTTGAGTGTGTGCT | AAAGTAAAACGTTATTTTAGCACGAAT | Start: 3 |
| Tm | 57.592 | 57.332 | End: 170 |
| Size | 22 | 27 | 168 |
| Primer 4 details |
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| Sequence | AGACTAGTTTTGAGTGTGTGCT | AAACGTTATTTTAGCACGAATAAATGA | Start: 3 |
| Tm | 57.592 | 57.171 | End: 164 |
| Size | 22 | 27 | 162 |
| Primer 5 details |
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| Sequence | TGTGCTAACATTTTGTTGAAGTGC | AAAAGTAAAACGTTATTTTAGCACGAA | Start: 19 |
| Tm | 59.197 | 57.499 | End: 171 |
| Size | 24 | 27 | 153 |
