ssr_primer
Primer Details for TRINITY_DN85380_c1_g1_i4 |
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| (T)16gtctctctttttaataca(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCGTGGTATTTGGCCTGT | GTGCTCTTCTGACCCGTCAA | Start: 2784 |
| Tm | 60.035 | 59.966 | End: 2927 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | CGGCTATCGTGGCTTCCTAG | GTGCTCTTCTGACCCGTCAA | Start: 2815 |
| Tm | 60.039 | 59.966 | End: 2927 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | CCAATTTGTCAACAGCGCGA | TAGGAGAGGGTACAGGGTGC | Start: 1469 |
| Tm | 60.041 | 60.032 | End: 1575 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
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| Sequence | TTTGTCCCTCGACTGTGAGC | GTAACATTGCGGTTGCTGCA | Start: 1920 |
| Tm | 59.966 | 60.04 | End: 2090 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
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| Sequence | TGCTTAGTCGTCAATGGCGT | ACAGGCCAAATACCACGGAG | Start: 2631 |
| Tm | 60.038 | 60.035 | End: 2803 |
| Size | 20 | 20 | 173 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCGTGGTATTTGGCCTGT | GTGCTCTTCTGACCCGTCAA | Start: 2784 |
| Tm | 60.035 | 59.966 | End: 2927 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | CGGCTATCGTGGCTTCCTAG | GTGCTCTTCTGACCCGTCAA | Start: 2815 |
| Tm | 60.039 | 59.966 | End: 2927 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | CCAATTTGTCAACAGCGCGA | TAGGAGAGGGTACAGGGTGC | Start: 1469 |
| Tm | 60.041 | 60.032 | End: 1575 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | TTTGTCCCTCGACTGTGAGC | GTAACATTGCGGTTGCTGCA | Start: 1920 |
| Tm | 59.966 | 60.04 | End: 2090 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TGCTTAGTCGTCAATGGCGT | ACAGGCCAAATACCACGGAG | Start: 2631 |
| Tm | 60.038 | 60.035 | End: 2803 |
| Size | 20 | 20 | 173 |
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