ssr_primer
Primer Details for TRINITY_DN85351_c0_g2_i1 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCAGGTCGCATCACTCGG | GCAGCATGGAATCCAGCATG | Start: 826 |
| Tm | 60.39 | 59.968 | End: 980 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGTCGCATCACTCGG | CGGTCTTGGCACTGAGAGTT | Start: 826 |
| Tm | 60.39 | 59.966 | End: 1014 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | GCAAAACCAAGGAGTCGCAA | Start: 81 |
| Tm | 59.89 | 59.616 | End: 270 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | TTGCAAAACCAAGGAGTCGC | Start: 81 |
| Tm | 59.89 | 59.616 | End: 272 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | GTGCCTGAACTTACGCGAGA | TGCGAATCTTACTCCCACGG | Start: 1734 |
| Tm | 60.388 | 59.825 | End: 1846 |
| Size | 20 | 20 | 113 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCAGGTCGCATCACTCGG | GCAGCATGGAATCCAGCATG | Start: 826 |
| Tm | 60.39 | 59.968 | End: 980 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGTCGCATCACTCGG | CGGTCTTGGCACTGAGAGTT | Start: 826 |
| Tm | 60.39 | 59.966 | End: 1014 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | GCAAAACCAAGGAGTCGCAA | Start: 81 |
| Tm | 59.89 | 59.616 | End: 270 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | TTGCAAAACCAAGGAGTCGC | Start: 81 |
| Tm | 59.89 | 59.616 | End: 272 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | GTGCCTGAACTTACGCGAGA | TGCGAATCTTACTCCCACGG | Start: 1734 |
| Tm | 60.388 | 59.825 | End: 1846 |
| Size | 20 | 20 | 113 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCAGGTCGCATCACTCGG | GCAGCATGGAATCCAGCATG | Start: 826 |
| Tm | 60.39 | 59.968 | End: 980 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGTCGCATCACTCGG | CGGTCTTGGCACTGAGAGTT | Start: 826 |
| Tm | 60.39 | 59.966 | End: 1014 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | GCAAAACCAAGGAGTCGCAA | Start: 81 |
| Tm | 59.89 | 59.616 | End: 270 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | AAGTGCTGAGGCTGAAGCTT | TTGCAAAACCAAGGAGTCGC | Start: 81 |
| Tm | 59.89 | 59.616 | End: 272 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | GTGCCTGAACTTACGCGAGA | TGCGAATCTTACTCCCACGG | Start: 1734 |
| Tm | 60.388 | 59.825 | End: 1846 |
| Size | 20 | 20 | 113 |
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