ssr_primer
Primer Details for TRINITY_DN85346_c1_g4_i5 |
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| (A)20caatgagcatttgcttggagtcagcaagataaagcagatgtctgttcattgacaatgcgccaaatttttttcactaagaatagtaaaattaa(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCCGTCCAGAGCAGAGA | AACGAAGAAAACAACCGCGG | Start: 1826 |
| Tm | 59.962 | 59.972 | End: 1990 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | CCGCGGTTGTTTTCTTCGTT | AATCAGCATGTCTGTCCCGG | Start: 1971 |
| Tm | 59.972 | 60.108 | End: 2165 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
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| Sequence | GGAGGATTTGCAGGCAGGAT | GTATTGCCTGCACCACCTCT | Start: 2002 |
| Tm | 60.106 | 60.035 | End: 2274 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
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| Sequence | TCTTCCGTCCAGAGCAGAGA | ATCCTGCCTGCAAATCCTCC | Start: 1826 |
| Tm | 59.962 | 60.106 | End: 2021 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
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| Sequence | GGAGGATTTGCAGGCAGGAT | AATCAGCATGTCTGTCCCGG | Start: 2002 |
| Tm | 60.106 | 60.108 | End: 2165 |
| Size | 20 | 20 | 164 |
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