ssr_primer
Primer Details for TRINITY_DN85303_c10_g1_i2 |
|---|
| (A)17ccttagatattttataaat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCAAGGTGTCATGGGTTT | GGTCCCGGTCCGTCAATAAC | Start: 1621 |
| Tm | 59.812 | 60.461 | End: 1727 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | AGCCAAGGTGTCATGGGTTT | GCGAGACGGAAAAGCCCATA | Start: 1621 |
| Tm | 59.812 | 60.461 | End: 1768 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | AGCCAAGGTGTCATGGGTTT | GTCCCGGTCCGTCAATAACT | Start: 1621 |
| Tm | 59.812 | 59.466 | End: 1726 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | ATTGCATCCCCCTGTGAAGT | TAGGAGACCATGCGACCAGA | Start: 2167 |
| Tm | 59.294 | 60.034 | End: 2269 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | AGCCAAGTGCCTCAAGGAAA | TTCGGAGCGATTTCCTTCCA | Start: 1771 |
| Tm | 59.814 | 59.389 | End: 1969 |
| Size | 20 | 20 | 199 |
(A)10t(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCAAGGTGTCATGGGTTT | GGTCCCGGTCCGTCAATAAC | Start: 1621 |
| Tm | 59.812 | 60.461 | End: 1727 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | AGCCAAGGTGTCATGGGTTT | GCGAGACGGAAAAGCCCATA | Start: 1621 |
| Tm | 59.812 | 60.461 | End: 1768 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | AGCCAAGGTGTCATGGGTTT | GTCCCGGTCCGTCAATAACT | Start: 1621 |
| Tm | 59.812 | 59.466 | End: 1726 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | ATTGCATCCCCCTGTGAAGT | TAGGAGACCATGCGACCAGA | Start: 2167 |
| Tm | 59.294 | 60.034 | End: 2269 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | AGCCAAGTGCCTCAAGGAAA | TTCGGAGCGATTTCCTTCCA | Start: 1771 |
| Tm | 59.814 | 59.389 | End: 1969 |
| Size | 20 | 20 | 199 |
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