ssr_primer
Primer Details for TRINITY_DN85302_c3_g1_i7 |
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| (A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCACCGAACAACACCT | TACGCCCTTACGTCAGAGGA | Start: 1985 |
| Tm | 60.034 | 60.035 | End: 2112 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | AAACCCACCGAACAACACCT | GTCCAGGGCTCATGTCGAAA | Start: 1985 |
| Tm | 60.034 | 60.036 | End: 2138 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | AGTCGAAGGCTCCAACAGTG | GTCCAGGGCTCATGTCGAAA | Start: 2022 |
| Tm | 59.966 | 60.036 | End: 2138 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | ATCGCATGCTACACACACCA | TCTTGAGCAAGGGCATCCAG | Start: 1224 |
| Tm | 60.036 | 60.035 | End: 1424 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
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| Sequence | TCCTCCAGAGCCACGAAAAC | ATTCAGTGAGTGCGGGAGTG | Start: 477 |
| Tm | 59.965 | 60.037 | End: 580 |
| Size | 20 | 20 | 104 |
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