ssr_primer
Primer Details for TRINITY_DN85289_c2_g2_i2 |
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| (A)21tggttatgtgccctcaatttaaaatgtccgagtaaaattattcaaaaccaaaacaatgtgtacgccccataaag(C)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGATCTTCCCGGCTACAAG | GACTTGGAGCAGTTGGACGA | Start: 473 |
| Tm | 60.249 | 59.966 | End: 665 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
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| Sequence | TCTGTGCATCTTGAGCTGCT | GACTTGGAGCAGTTGGACGA | Start: 397 |
| Tm | 59.676 | 59.966 | End: 665 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
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| Sequence | TGGCAAGATGACACTGTGCA | GGCGATGCATAAAAACCGGG | Start: 164 |
| Tm | 60.179 | 60.249 | End: 263 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | TGGCAAGATGACACTGTGCA | AAAGCTAGAAGGTGCGCGAA | Start: 164 |
| Tm | 60.179 | 60.32 | End: 283 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | ATCCGATCTTCCCGGCTACA | GACTTGGAGCAGTTGGACGA | Start: 471 |
| Tm | 60.469 | 59.966 | End: 665 |
| Size | 20 | 20 | 195 |
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