ssr_primer
Primer Details for TRINITY_DN85262_c0_g2_i7 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGCGTTGTCCACTGAACT | TCTTCCCGAACTCCTTGTGC | Start: 140 |
| Tm | 59.966 | 59.965 | End: 410 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
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| Sequence | GCACAAGGAGTTCGGGAAGA | CCTGGGAGTTATTGGCCGTT | Start: 391 |
| Tm | 59.965 | 60.034 | End: 527 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | GCACAAGGAGTTCGGGAAGA | CAGGCCCTGGGAGTTATTGG | Start: 391 |
| Tm | 59.965 | 60.107 | End: 532 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | CCTTGGATTGCCGACCATCT | TCTTCCCGAACTCCTTGTGC | Start: 166 |
| Tm | 60.107 | 59.965 | End: 410 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
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| Sequence | TCCTTGGATTGCCGACCATC | TCTTCCCGAACTCCTTGTGC | Start: 165 |
| Tm | 60.107 | 59.965 | End: 410 |
| Size | 20 | 20 | 246 |
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