ssr_primer
Primer Details for TRINITY_DN85261_c7_g2_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTAAACGGCCGTAGAG | CCAGAGGTTGCTCCCGAAAT | Start: 354 |
| Tm | 59.827 | 60.035 | End: 505 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
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| Sequence | ACAGCTAAACGGCCGTAGAG | AGAGGTTGCTCCCGAAATCG | Start: 354 |
| Tm | 59.827 | 60.108 | End: 503 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
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| Sequence | ACAGCTAAACGGCCGTAGAG | GAGGTTGCTCCCGAAATCGA | Start: 354 |
| Tm | 59.827 | 60.108 | End: 502 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
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| Sequence | ATTTCGGGAGCAACCTCTGG | GCGTCATAGCCAGCATTGAC | Start: 486 |
| Tm | 60.035 | 59.694 | End: 648 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | ATTTCGGGAGCAACCTCTGG | TACGGTCTCGGGTTCCAAAC | Start: 486 |
| Tm | 60.035 | 59.682 | End: 619 |
| Size | 20 | 20 | 134 |
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