ssr_primer
Primer Details for TRINITY_DN85255_c3_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCAAGAGCTATCGTTGT | TGGTTGAAACGGGTGGTTCT | Start: 22 |
| Tm | 60.39 | 59.744 | End: 171 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | TGCGCAAGAGCTATCGTTGT | TGCACATAGCTCCGTTTGGA | Start: 22 |
| Tm | 60.39 | 59.677 | End: 240 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | TCCAAACGGAGCTATGTGCA | GGGTATCCCAATTTGCCGGT | Start: 221 |
| Tm | 59.677 | 60.397 | End: 450 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | AGAACCACCCGTTTCAACCA | AGGGCTCATGTCTCATTGCA | Start: 152 |
| Tm | 59.744 | 59.378 | End: 256 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | AGAACCACCCGTTTCAACCA | GGGCTCATGTCTCATTGCAC | Start: 152 |
| Tm | 59.744 | 59.263 | End: 255 |
| Size | 20 | 20 | 104 |
(AGATA)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCAAGAGCTATCGTTGT | TGGTTGAAACGGGTGGTTCT | Start: 22 |
| Tm | 60.39 | 59.744 | End: 171 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | TGCGCAAGAGCTATCGTTGT | TGCACATAGCTCCGTTTGGA | Start: 22 |
| Tm | 60.39 | 59.677 | End: 240 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | TCCAAACGGAGCTATGTGCA | GGGTATCCCAATTTGCCGGT | Start: 221 |
| Tm | 59.677 | 60.397 | End: 450 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | AGAACCACCCGTTTCAACCA | AGGGCTCATGTCTCATTGCA | Start: 152 |
| Tm | 59.744 | 59.378 | End: 256 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | AGAACCACCCGTTTCAACCA | GGGCTCATGTCTCATTGCAC | Start: 152 |
| Tm | 59.744 | 59.263 | End: 255 |
| Size | 20 | 20 | 104 |
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