ssr_primer
Primer Details for TRINITY_DN85221_c0_g1_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGAAGTGGGGCAACTACC | TGATAATCGCGCCTCAGCAT | Start: 585 |
| Tm | 60.035 | 59.966 | End: 755 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | ATGCTGATAATCGCGCCTCA | Start: 585 |
| Tm | 60.035 | 59.966 | End: 759 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | GCTGCCATACCCTTGTGAGT | Start: 585 |
| Tm | 60.035 | 60.035 | End: 700 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | GCCCCAGAAGTGCTAGAAGG | GCTGCCATACCCTTGTGAGT | Start: 435 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | GGCCCCAGAAGTGCTAGAAG | GCTGCCATACCCTTGTGAGT | Start: 434 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 267 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGAAGTGGGGCAACTACC | TGATAATCGCGCCTCAGCAT | Start: 585 |
| Tm | 60.035 | 59.966 | End: 755 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | ATGCTGATAATCGCGCCTCA | Start: 585 |
| Tm | 60.035 | 59.966 | End: 759 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | GCTGCCATACCCTTGTGAGT | Start: 585 |
| Tm | 60.035 | 60.035 | End: 700 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | GCCCCAGAAGTGCTAGAAGG | GCTGCCATACCCTTGTGAGT | Start: 435 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | GGCCCCAGAAGTGCTAGAAG | GCTGCCATACCCTTGTGAGT | Start: 434 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 267 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGAAGTGGGGCAACTACC | TGATAATCGCGCCTCAGCAT | Start: 585 |
| Tm | 60.035 | 59.966 | End: 755 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | ATGCTGATAATCGCGCCTCA | Start: 585 |
| Tm | 60.035 | 59.966 | End: 759 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | GAGGAAGTGGGGCAACTACC | GCTGCCATACCCTTGTGAGT | Start: 585 |
| Tm | 60.035 | 60.035 | End: 700 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | GCCCCAGAAGTGCTAGAAGG | GCTGCCATACCCTTGTGAGT | Start: 435 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | GGCCCCAGAAGTGCTAGAAG | GCTGCCATACCCTTGTGAGT | Start: 434 |
| Tm | 60.107 | 60.035 | End: 700 |
| Size | 20 | 20 | 267 |
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