ssr_primer
Primer Details for TRINITY_DN85210_c3_g5_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCATCTGTGCCAAACTCG | CGTTCGGTGGAAATCCGTTG | Start: 137 |
| Tm | 59.555 | 59.835 | End: 308 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
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| Sequence | GCTCATCTGTGCCAAACTCG | GGTGGAAATCCGTTGGCTTG | Start: 137 |
| Tm | 59.555 | 59.756 | End: 303 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | CCAAACTCGGCCAATCCAAG | CGTTCGGTGGAAATCCGTTG | Start: 148 |
| Tm | 59.472 | 59.835 | End: 308 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | GCTCATCTGTGCCAAACTCG | ACGTTCGGTGGAAATCCGTT | Start: 137 |
| Tm | 59.555 | 60.25 | End: 309 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | CCAAACTCGGCCAATCCAAG | GGTGGAAATCCGTTGGCTTG | Start: 148 |
| Tm | 59.472 | 59.756 | End: 303 |
| Size | 20 | 20 | 156 |
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