ssr_primer
Primer Details for TRINITY_DN85150_c3_g2_i6 |
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| (A)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCCATGGGGTCCCTTTC | CTGGCGGAAAGACCATCCTT | Start: 2667 |
| Tm | 60.033 | 60.035 | End: 2785 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | TTTGTACGAGCCACCTTCCC | CTGGCGGAAAGACCATCCTT | Start: 2653 |
| Tm | 59.964 | 60.035 | End: 2785 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
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| Sequence | GGGGTCCCTTTCTTTCCAGG | CTGGCGGAAAGACCATCCTT | Start: 2675 |
| Tm | 59.96 | 60.035 | End: 2785 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
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| Sequence | AGGATGGAAGAGTGGAGGCT | CTGGCGGAAAGACCATCCTT | Start: 2578 |
| Tm | 59.956 | 60.035 | End: 2785 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
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| Sequence | AGGATGGAAGAGTGGAGGCT | CCTGGAAAGAAAGGGACCCC | Start: 2578 |
| Tm | 59.956 | 59.96 | End: 2694 |
| Size | 20 | 20 | 117 |
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