ssr_primer
Primer Details for TRINITY_DN85147_c1_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTTTCCAAGTTACCAAATGT | ACTCAATGGACAGCCTTTTCA | Start: 27 |
| Tm | 57.13 | 57.772 | End: 155 |
| Size | 23 | 21 | 129 |
| Primer 2 details |
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| Sequence | AGCTTTTCCAAGTTACCAAATGT | AACTCAATGGACAGCCTTTTCA | Start: 27 |
| Tm | 57.13 | 58.435 | End: 156 |
| Size | 23 | 22 | 130 |
| Primer 3 details |
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| Sequence | AGCTTTTCCAAGTTACCAAATGT | ACTCAATGGACAGCCTTTTCAT | Start: 27 |
| Tm | 57.13 | 58.229 | End: 155 |
| Size | 23 | 22 | 129 |
| Primer 4 details |
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| Sequence | AGCTTTTCCAAGTTACCAAATGT | AAACTCAATGGACAGCCTTTTCA | Start: 27 |
| Tm | 57.13 | 59.04 | End: 157 |
| Size | 23 | 23 | 131 |
| Primer 5 details |
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| Sequence | TGAAAAGGCTGTCCATTGAGT | TTTACACGTTTTTCCTCATGAAGT | Start: 135 |
| Tm | 57.772 | 57.313 | End: 235 |
| Size | 21 | 24 | 101 |
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