ssr_primer
Primer Details for TRINITY_DN85133_c1_g3_i1 |
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| (C)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTCCCCTTTACCCTTGCG | ATAACGAATGGGCAGACCGG | Start: 9 |
| Tm | 60.108 | 60.179 | End: 200 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
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| Sequence | GCTCTCCCCTTTACCCTTGC | ATAACGAATGGGCAGACCGG | Start: 8 |
| Tm | 60.394 | 60.179 | End: 200 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
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| Sequence | TCTCTCTAGTTCCGGACGGC | ATAACGAATGGGCAGACCGG | Start: 83 |
| Tm | 60.747 | 60.179 | End: 200 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | CTCTCCCCTTTACCCTTGCG | GGGGGTGAGAAATGCGGAA | Start: 9 |
| Tm | 60.108 | 60.001 | End: 237 |
| Size | 20 | 19 | 229 |
| Primer 5 details |
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| Sequence | CTCTCCCCTTTACCCTTGCG | GGGGGTGAGAAATGCGGAAG | Start: 9 |
| Tm | 60.108 | 61.032 | End: 237 |
| Size | 20 | 20 | 229 |
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