ssr_primer
Primer Details for TRINITY_DN85106_c4_g2_i2 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTGGGGAGGATCGTTGGG | CGTGGGTTCGGTTAAGTCCA | Start: 1602 |
| Tm | 59.741 | 59.966 | End: 1881 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | GAAAGGCTGGGGAGCTGAAT | GCGCAAAGTTGGTGCTTCAT | Start: 42 |
| Tm | 60.034 | 60.319 | End: 317 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | CAAATTCCATGGGGTGCTGC | TCACCTCAATGGCGTTACGT | Start: 960 |
| Tm | 60.108 | 59.681 | End: 1129 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | AACTTTTCCCAAGCCTCCCC | CCCAAATCCCCCTCAATCGT | Start: 1375 |
| Tm | 60.179 | 59.741 | End: 1608 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | AACTTTTCCCAAGCCTCCCC | CCCAACGATCCTCCCCAAAT | Start: 1375 |
| Tm | 60.179 | 59.741 | End: 1621 |
| Size | 20 | 20 | 247 |
(ATT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTGGGGAGGATCGTTGGG | CGTGGGTTCGGTTAAGTCCA | Start: 1602 |
| Tm | 59.741 | 59.966 | End: 1881 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | GAAAGGCTGGGGAGCTGAAT | GCGCAAAGTTGGTGCTTCAT | Start: 42 |
| Tm | 60.034 | 60.319 | End: 317 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | CAAATTCCATGGGGTGCTGC | TCACCTCAATGGCGTTACGT | Start: 960 |
| Tm | 60.108 | 59.681 | End: 1129 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | AACTTTTCCCAAGCCTCCCC | CCCAAATCCCCCTCAATCGT | Start: 1375 |
| Tm | 60.179 | 59.741 | End: 1608 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | AACTTTTCCCAAGCCTCCCC | CCCAACGATCCTCCCCAAAT | Start: 1375 |
| Tm | 60.179 | 59.741 | End: 1621 |
| Size | 20 | 20 | 247 |
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