ssr_primer
Primer Details for TRINITY_DN85077_c2_g3_i10 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGCCCAAATCACGCCC | CTCCCTTGGGACCCTGAGTA | Start: 319 |
| Tm | 60.036 | 59.957 | End: 435 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | GCTCGTCAGCTTGGAAGTCT | CTCCCTTGGGACCCTGAGTA | Start: 253 |
| Tm | 60.038 | 59.957 | End: 435 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | GTTTTCACCGAACCCGCAAA | AGACTTCCAAGCTGACGAGC | Start: 31 |
| Tm | 59.899 | 60.038 | End: 272 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TAGTTTTCACCGAACCCGCA | AGACTTCCAAGCTGACGAGC | Start: 29 |
| Tm | 59.894 | 60.038 | End: 272 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | GGGACGAGAAGGGTGTTACG | GGGCGTGATTTGGGCAAATT | Start: 133 |
| Tm | 60.109 | 60.036 | End: 338 |
| Size | 20 | 20 | 206 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGCCCAAATCACGCCC | CTCCCTTGGGACCCTGAGTA | Start: 319 |
| Tm | 60.036 | 59.957 | End: 435 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | GCTCGTCAGCTTGGAAGTCT | CTCCCTTGGGACCCTGAGTA | Start: 253 |
| Tm | 60.038 | 59.957 | End: 435 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | GTTTTCACCGAACCCGCAAA | AGACTTCCAAGCTGACGAGC | Start: 31 |
| Tm | 59.899 | 60.038 | End: 272 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TAGTTTTCACCGAACCCGCA | AGACTTCCAAGCTGACGAGC | Start: 29 |
| Tm | 59.894 | 60.038 | End: 272 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | GGGACGAGAAGGGTGTTACG | GGGCGTGATTTGGGCAAATT | Start: 133 |
| Tm | 60.109 | 60.036 | End: 338 |
| Size | 20 | 20 | 206 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGCCCAAATCACGCCC | CTCCCTTGGGACCCTGAGTA | Start: 319 |
| Tm | 60.036 | 59.957 | End: 435 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | GCTCGTCAGCTTGGAAGTCT | CTCCCTTGGGACCCTGAGTA | Start: 253 |
| Tm | 60.038 | 59.957 | End: 435 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | GTTTTCACCGAACCCGCAAA | AGACTTCCAAGCTGACGAGC | Start: 31 |
| Tm | 59.899 | 60.038 | End: 272 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TAGTTTTCACCGAACCCGCA | AGACTTCCAAGCTGACGAGC | Start: 29 |
| Tm | 59.894 | 60.038 | End: 272 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | GGGACGAGAAGGGTGTTACG | GGGCGTGATTTGGGCAAATT | Start: 133 |
| Tm | 60.109 | 60.036 | End: 338 |
| Size | 20 | 20 | 206 |
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