ssr_primer
Primer Details for TRINITY_DN85053_c0_g1_i2 |
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| (T)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACCATGGCATCCAGTGA | TGCGCTAGTCACAGAATCGT | Start: 8 |
| Tm | 59.006 | 59.474 | End: 190 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | TGAATGATGTCATACGCCGGT | TGCGCTAGTCACAGAATCGT | Start: 25 |
| Tm | 59.862 | 59.474 | End: 190 |
| Size | 21 | 20 | 166 |
| Primer 3 details |
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| Sequence | ACCATGGCATCCAGTGAATGA | TGCGCTAGTCACAGAATCGT | Start: 11 |
| Tm | 59.714 | 59.474 | End: 190 |
| Size | 21 | 20 | 180 |
| Primer 4 details |
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| Sequence | AGAACCATGGCATCCAGTGAA | TGCGCTAGTCACAGAATCGT | Start: 8 |
| Tm | 59.645 | 59.474 | End: 190 |
| Size | 21 | 20 | 183 |
| Primer 5 details |
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| Sequence | AAGAACCATGGCATCCAGTGA | TGCGCTAGTCACAGAATCGT | Start: 7 |
| Tm | 59.645 | 59.474 | End: 190 |
| Size | 21 | 20 | 184 |
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