ssr_primer
Primer Details for TRINITY_DN85040_c2_g1_i4 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGACTGCGCGACAAATTAG | TGAATAAACCTGGCCAGCGT | Start: 0 |
| Tm | 59.975 | 59.962 | End: 263 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | GGTTTTCGCACGGCATTGAT | CACTCGCGCAATGTGTGAAA | Start: 3441 |
| Tm | 60.109 | 60.041 | End: 3540 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TCACACAATGACGGTGCAGT | GCTGCTGCGTTATTCTGCTC | Start: 1813 |
| Tm | 60.179 | 59.973 | End: 1972 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GCCTCTCCCTTTTCTAGCCG | CACTCGCGCAATGTGTGAAA | Start: 3361 |
| Tm | 60.179 | 60.041 | End: 3540 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | GCCTCTCCCTTTTCTAGCCG | GGGGTGGAAACTTGGGTTCA | Start: 3361 |
| Tm | 60.179 | 60.106 | End: 3487 |
| Size | 20 | 20 | 127 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGACTGCGCGACAAATTAG | TGAATAAACCTGGCCAGCGT | Start: 0 |
| Tm | 59.975 | 59.962 | End: 263 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | GGTTTTCGCACGGCATTGAT | CACTCGCGCAATGTGTGAAA | Start: 3441 |
| Tm | 60.109 | 60.041 | End: 3540 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TCACACAATGACGGTGCAGT | GCTGCTGCGTTATTCTGCTC | Start: 1813 |
| Tm | 60.179 | 59.973 | End: 1972 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GCCTCTCCCTTTTCTAGCCG | CACTCGCGCAATGTGTGAAA | Start: 3361 |
| Tm | 60.179 | 60.041 | End: 3540 |
| Size | 20 | 20 | 180 |
| Primer 5 details |
|---|
| Sequence | GCCTCTCCCTTTTCTAGCCG | GGGGTGGAAACTTGGGTTCA | Start: 3361 |
| Tm | 60.179 | 60.106 | End: 3487 |
| Size | 20 | 20 | 127 |
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