ssr_primer
Primer Details for TRINITY_DN85033_c0_g1_i1 |
|---|
| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTTTCATCGACGGGGTT | ATCTCTGCAAACGCGAGGAA | Start: 3289 |
| Tm | 60.035 | 60.037 | End: 3404 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | ACATCCGCTCTACCAGGAGT | TTTGAGGGAACGACGTCGAG | Start: 640 |
| Tm | 60.033 | 60.04 | End: 860 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | GGCTCAAACCCCGATCTTCA | TCTGTCTGCTATGCAACGCA | Start: 670 |
| Tm | 60.035 | 60.037 | End: 921 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | TGACATCCGCTCTACCAGGA | TTTGAGGGAACGACGTCGAG | Start: 638 |
| Tm | 60.034 | 60.04 | End: 860 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | GGCTCAAACCCCGATCTTCA | TTTGAGGGAACGACGTCGAG | Start: 670 |
| Tm | 60.035 | 60.04 | End: 860 |
| Size | 20 | 20 | 191 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTTTCATCGACGGGGTT | ATCTCTGCAAACGCGAGGAA | Start: 3289 |
| Tm | 60.035 | 60.037 | End: 3404 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | ACATCCGCTCTACCAGGAGT | TTTGAGGGAACGACGTCGAG | Start: 640 |
| Tm | 60.033 | 60.04 | End: 860 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | GGCTCAAACCCCGATCTTCA | TCTGTCTGCTATGCAACGCA | Start: 670 |
| Tm | 60.035 | 60.037 | End: 921 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | TGACATCCGCTCTACCAGGA | TTTGAGGGAACGACGTCGAG | Start: 638 |
| Tm | 60.034 | 60.04 | End: 860 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | GGCTCAAACCCCGATCTTCA | TTTGAGGGAACGACGTCGAG | Start: 670 |
| Tm | 60.035 | 60.04 | End: 860 |
| Size | 20 | 20 | 191 |
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