ssr_primer
Primer Details for TRINITY_DN85010_c1_g1_i2 |
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| (G)13cttgcaatacatgaagaaatgcctgaaccccaaaatttgacccttgttttgtaggtctatcaacaccaggaatcatgagtcgagcaagggtttgctt(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGTTGGGGTTCTTCCGTA | GGCTTCTCTGATGGCGGTTA | Start: 890 |
| Tm | 59.964 | 59.822 | End: 1016 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | TCCCCTGGCATTTCTTTGCA | Start: 890 |
| Tm | 59.964 | 60.179 | End: 1036 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
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| Sequence | TTGTGTGCCTGTGTCTCCTG | TGAAGGCTGCAAGATGAGCA | Start: 1618 |
| Tm | 60.179 | 59.963 | End: 1875 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCTGGCATTTCTTTGCAGG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1034 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCCTGGCATTTCTTTGCAG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1035 |
| Size | 20 | 20 | 146 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGTTGGGGTTCTTCCGTA | GGCTTCTCTGATGGCGGTTA | Start: 890 |
| Tm | 59.964 | 59.822 | End: 1016 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | TCCCCTGGCATTTCTTTGCA | Start: 890 |
| Tm | 59.964 | 60.179 | End: 1036 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TTGTGTGCCTGTGTCTCCTG | TGAAGGCTGCAAGATGAGCA | Start: 1618 |
| Tm | 60.179 | 59.963 | End: 1875 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCTGGCATTTCTTTGCAGG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1034 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCCTGGCATTTCTTTGCAG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1035 |
| Size | 20 | 20 | 146 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGTTGGGGTTCTTCCGTA | GGCTTCTCTGATGGCGGTTA | Start: 890 |
| Tm | 59.964 | 59.822 | End: 1016 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | TCCCCTGGCATTTCTTTGCA | Start: 890 |
| Tm | 59.964 | 60.179 | End: 1036 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TTGTGTGCCTGTGTCTCCTG | TGAAGGCTGCAAGATGAGCA | Start: 1618 |
| Tm | 60.179 | 59.963 | End: 1875 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCTGGCATTTCTTTGCAGG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1034 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GCAGTTGGGGTTCTTCCGTA | CCCCTGGCATTTCTTTGCAG | Start: 890 |
| Tm | 59.964 | 59.752 | End: 1035 |
| Size | 20 | 20 | 146 |
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