ssr_primer
Primer Details for TRINITY_DN84996_c1_g1_i6 |
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| (A)52: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACTCGCTTCTTCGTCCAGT | GGATTTCGTCTCCACTGCCA | Start: 3079 |
| Tm | 60.039 | 60.036 | End: 3225 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
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| Sequence | CACTCGCTTCTTCGTCCAGT | ACTGCCAGCGGAAGAAATCA | Start: 3079 |
| Tm | 60.039 | 59.963 | End: 3212 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | GGGCTTCAGATCAGGTGCTT | CGGTTCTCTCGTCTCTGAGC | Start: 967 |
| Tm | 60.035 | 59.902 | End: 1167 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
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| Sequence | GGAGTCAGCGTTGGTAACCA | GCGGGCAAGCAGATTTTAGG | Start: 1571 |
| Tm | 59.966 | 59.899 | End: 1840 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | GGGCTTCAGATCAGGTGCTT | GATCCAGTGGCAGAGACGAG | Start: 967 |
| Tm | 60.035 | 59.898 | End: 1146 |
| Size | 20 | 20 | 180 |
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