ssr_primer
Primer Details for TRINITY_DN84995_c0_g1_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCTCTAATCCATCCCCA | GCCTGAGTGGTTGCAATTGG | Start: 1193 |
| Tm | 60.029 | 60.038 | End: 1457 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
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| Sequence | ATCCATCCCCAGCATTTGCA | GCCTGAGTGGTTGCAATTGG | Start: 1202 |
| Tm | 60.032 | 60.038 | End: 1457 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | CTGAAATCAACCCTCCGCCT | ACTCTTGAGCAGTGAAGGGC | Start: 620 |
| Tm | 60.035 | 59.964 | End: 760 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
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| Sequence | CTGAAATCAACCCTCCGCCT | TCTTCTCTTTTCGCCCACCC | Start: 620 |
| Tm | 60.035 | 59.964 | End: 797 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | TGAAATCAACCCTCCGCCTC | ACTCTTGAGCAGTGAAGGGC | Start: 621 |
| Tm | 60.035 | 59.964 | End: 760 |
| Size | 20 | 20 | 140 |
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