ssr_primer
Primer Details for TRINITY_DN84980_c7_g3_i4 |
|---|
| (T)11(A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGTACTGATGACGATGA | TCTGAGCTGGATCCAGACGA | Start: 692 |
| Tm | 59.971 | 60.034 | End: 849 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | TGAGGTGGATACGGACCCAT | Start: 3558 |
| Tm | 59.967 | 60.032 | End: 3756 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | CCTTCACGACTTCCTCCACC | TGAGGTGGATACGGACCCAT | Start: 3554 |
| Tm | 60.037 | 60.032 | End: 3756 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | GATGCGAAGGTTGAGGTGGA | Start: 3558 |
| Tm | 59.967 | 60.036 | End: 3767 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | TGACTCCCAACCAAGGATGC | TGAGGTGGATACGGACCCAT | Start: 3490 |
| Tm | 59.962 | 60.032 | End: 3756 |
| Size | 20 | 20 | 267 |
(TGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGTACTGATGACGATGA | TCTGAGCTGGATCCAGACGA | Start: 692 |
| Tm | 59.971 | 60.034 | End: 849 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | TGAGGTGGATACGGACCCAT | Start: 3558 |
| Tm | 59.967 | 60.032 | End: 3756 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | CCTTCACGACTTCCTCCACC | TGAGGTGGATACGGACCCAT | Start: 3554 |
| Tm | 60.037 | 60.032 | End: 3756 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | GATGCGAAGGTTGAGGTGGA | Start: 3558 |
| Tm | 59.967 | 60.036 | End: 3767 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | TGACTCCCAACCAAGGATGC | TGAGGTGGATACGGACCCAT | Start: 3490 |
| Tm | 59.962 | 60.032 | End: 3756 |
| Size | 20 | 20 | 267 |
(T)10ctctctc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGTACTGATGACGATGA | TCTGAGCTGGATCCAGACGA | Start: 692 |
| Tm | 59.971 | 60.034 | End: 849 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | TGAGGTGGATACGGACCCAT | Start: 3558 |
| Tm | 59.967 | 60.032 | End: 3756 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | CCTTCACGACTTCCTCCACC | TGAGGTGGATACGGACCCAT | Start: 3554 |
| Tm | 60.037 | 60.032 | End: 3756 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CACGACTTCCTCCACCGAAA | GATGCGAAGGTTGAGGTGGA | Start: 3558 |
| Tm | 59.967 | 60.036 | End: 3767 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | TGACTCCCAACCAAGGATGC | TGAGGTGGATACGGACCCAT | Start: 3490 |
| Tm | 59.962 | 60.032 | End: 3756 |
| Size | 20 | 20 | 267 |
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