ssr_primer
Primer Details for TRINITY_DN84961_c5_g1_i13 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAACACTCGTTCCTCCGA | TCGATTGTGTTTGGCCTCGA | Start: 4865 |
| Tm | 59.967 | 59.966 | End: 5022 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
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| Sequence | ACTGCATGAATCCAACCGGT | TCGGAGGAACGAGTGTTTGG | Start: 4671 |
| Tm | 59.962 | 59.967 | End: 4884 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
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| Sequence | GGCCCAATCGTCTGTGTACA | TGCTGTGTGGGTCTGTGTTT | Start: 5444 |
| Tm | 60.037 | 60.035 | End: 5588 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
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| Sequence | GTTGGTGATGCTGGCTAGGT | TGCCACGTTCCTAAGCCAAT | Start: 220 |
| Tm | 60.035 | 59.962 | End: 431 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
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| Sequence | TGTTGGTGATGCTGGCTAGG | TGCCACGTTCCTAAGCCAAT | Start: 219 |
| Tm | 60.035 | 59.962 | End: 431 |
| Size | 20 | 20 | 213 |
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